Influence of surface free energy of denture base and liner materials on Candida albicans biofilms
Article first published online: 11 JAN 2014
© 2014 Wiley Publishing Asia Pty Ltd
Journal of Investigative and Clinical Dentistry
How to Cite
da Silva, W. J., Leal, C. M. B., Viu, F. C., Gonçalves, L. M., Barbosa, C. M. R. and Del Bel Cury, A. A. (2014), Influence of surface free energy of denture base and liner materials on Candida albicans biofilms. Journal of Investigative and Clinical Dentistry. doi: 10.1111/jicd.12079
- Article first published online: 11 JAN 2014
- Manuscript Accepted: 20 AUG 2013
- Manuscript Received: 21 APR 2013
- FAPESP. Grant Number: 2010/02091-5
- acrylic resins;
- Candida albicans;
- surface properties
This study aimed to evaluate the influence of surface free energy (SFE) of denture base and liner materials on Candida albicans biofilm development.
Discs were fabricated using poly(methyl methacrylate) acrylic resin and poly(ethyl methacrylate) denture liner, according to the manufacturers' instructions. For SFE test, discs were pellicle-coated with saliva alone, saliva + blood plasma, or blood plasma alone. Candida albicans biofilms were allowed to form on pellicle-coated discs for 48 h. Biofilms were evaluated for cell counts, metabolic activity, and structural characteristics at adhesion phase (after 1.5 h of development) and at biofilm maturity (after 48 h of development). Data were analyzed by anova and Tukey tests using a significance level of 5%.
Saliva + blood plasma pellicles had a higher SFE compared to pellicles of saliva or blood plasma alone (P < 0.001). Differences in SFE by pellicle-coating did not affect the cell counts, metabolic activity, or structure at the adhesion phase (P > 0.05). In contrast, the presence of blood plasma resulted in higher cell counts, biovolume, and thickness of mature biofilms on both materials (P < 0.001).
Increases in SFE from pellicle-coating leads to robust mature C. albicans biofilms on both denture materials.