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Confocal analysis of the exopolysaccharide matrix of Candida albicans biofilms

Authors

  • Letícia M. Gonçalves,

    1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
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  • Altair A. Del Bel Cury,

    1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
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  • Andréa A. de Vasconcellos,

    1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
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  • Jaime A. Cury,

    1. Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
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  • Wander J. da Silva

    Corresponding author
    1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil
    • Correspondence

      Dr W. J. da Silva, Department of Prosthodontics and Periodontology; Piracicaba Dental School, University of Campinas. Avenida Limeira, 901 Piracicaba, São Paulo 13414-903, Brazil.

      Tel: +55-19-2106-5294

      Fax: +55-19-2106-5211

      Email: wanderjose@fop.unicamp.br

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Abstract

Aim

Confocal laser-scanning microscopy (CLSM) was carried out to investigate the exopolysaccharide matrix of Candida albicans (C. albicans) biofilms developed on denture material under dietary carbohydrate exposure.

Methods

Biofilms were developed on poly(methyl methacrylate) discs in culture media without (control) or with supplementation by glucose or sucrose for 72 h. For the CLSM analysis, biofilms were labeled with concanavalin A (ConA) during its development. Afterwards, biofilms were also labeled with SYTO-9. To confirm the results, the matrix was investigated by the phenol-sulfuric method. Data were analyzed by anova, followed by Tukey's test, with the level of significance set at 5%.

Results

The use of ConA during biofilm development provided effective labeling of the exopolysaccharide matrix. The exposure to sucrose resulted in biofilms with the highest exopolysaccharide matrix biovolume (< 0.05). The characterization obtained by CLSM was confirmed by the phenol-sulfuric method.

Conclusion

Confocal laser-scanning microscopy was found to be an effective tool for investigating the exopolysaccharide matrix of C. albicans biofilms, and exposure to sucrose resulted in increased matrix production.

Ancillary