This Special Issue focuses on plant organelle biology. Papers included in this issue provide updates on the latest research developments in organelle extensions, division, movement, trafficking, and more. The cover picture was taken from a review paper from Worden et al. (875–886) that summarizes the endomembrane trafficking pathways in cell wall deposition. Endomembrane trafficking facilitates the transport of polysaccharides, structural proteins and biosynthetic enzymes from the Golgi apparatus to the plasma membrane and the apoplast (image provided by Park and Drakakaki).