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Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in ArabidopsisF
Article first published online: 21 JAN 2013
© 2013 Institute of Botany, Chinese Academy of Sciences
Journal of Integrative Plant Biology
Special Issue: Ubiquitination and Sumoylation
Volume 55, Issue 1, pages 75–82, January 2013
How to Cite
Tomanov, K., Hardtke, C., Budhiraja, R., Hermkes, R., Coupland, G. and Bachmair, A. (2013), Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis. Journal of Integrative Plant Biology, 55: 75–82. doi: 10.1111/jipb.12016
- Issue published online: 21 JAN 2013
- Article first published online: 21 JAN 2013
- Accepted manuscript online: 3 DEC 2012 11:32PM EST
- Received 8 Sept. 2012 Accepted 19 Nov. 2012
Figure S1. Fresh weight of Arabidopsis plants of different genotypes grown in the presence or absence of ammonium sulfate as a source of reduced nitrogen.
Seeds of genotype siz1 (A), seeds over-expressing SCE(C94S) (B), or seeds over-expressing WT SCE (C) were sown on soil containing nitrate fertilizer. A parallel set of pots was prepared in identical manner, but was watered with 5 mM (NH4)2SO4 instead of tap water. After three weeks, above ground parts of 40 plants were harvested and used to determine fresh weight. The number of plants is on the x-axis, their weight (in mg per plantlet) on the y-axis. (D), Average weight of plantlets in presence or in absence of (NH4)2SO4. Note that WT SCE overexpressing plants grow better with nitrate as the sole nitrogen source, whereas siz1 plants and SCE(C94S) overexpressing plants profit from the presence of reduced nitrogen. The SCE(C94S) expressing line chosen for this experiment does not display greatly reduced growth (as observed for most lines used in gene expression and SUMO conjugate studies), which is indicative of only moderate over-expression.
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