Three-dimensional imaging of whole mouse models: comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging

Authors

  • A. TAPFER,

    Corresponding author
    1. Department of Physics and Institute of Medical Engineering, Technische Universität München, Munich, Germany
    • Correspondence to: Arne Tapfer, Physics Department, Technische Universität München, , James-Franck-Street, 85748 Garching, Germany. Tel: +49-89-289-12552; fax: +49-289-12548; e-mail: arne.tapfer@tum.de, franz.pfeiffer@tum.de

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  • M. BECH,

    1. Department of Physics and Institute of Medical Engineering, Technische Universität München, Munich, Germany
    2. Medical Radiation Physics, Clinical Sciences, Lund University, Lund, Sweden
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  • I. ZANETTE,

    1. Department of Physics and Institute of Medical Engineering, Technische Universität München, Munich, Germany
    2. European Synchrotron Radiation Facility, Grenoble, France
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  • P. SYMVOULIDIS,

    1. Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany
    2. Chair for Biological Imaging, Technische Universität München, Munich, Germany
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  • S. STANGL,

    1. Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany
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  • G. MULTHOFF,

    1. Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany
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  • M. MOLLS,

    1. Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany
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  • V. NTZIACHRISTOS,

    1. Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg, Germany
    2. Chair for Biological Imaging, Technische Universität München, Munich, Germany
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  • F. PFEIFFER

    1. Department of Physics and Institute of Medical Engineering, Technische Universität München, Munich, Germany
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Summary

In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft-tissue contrast is strongly enhanced in the X-ray phase images compared to the attenuation-based images. This identifies phase-contrast micro-CT as a powerful tool for the study of small animal disease models.

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