Loss of bone marrow NK cells during SIV infection is associated with increased turnover rates and cytotoxicity but not changes in trafficking
Version of Record online: 30 JUL 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Journal of Medical Primatology
Volume 42, Issue 5, pages 230–236, October 2013
How to Cite
Li, H., Evans, T. I. and Reeves, R. K. (2013), Loss of bone marrow NK cells during SIV infection is associated with increased turnover rates and cytotoxicity but not changes in trafficking. Journal of Medical Primatology, 42: 230–236. doi: 10.1111/jmp.12063
- Issue online: 12 SEP 2013
- Version of Record online: 30 JUL 2013
- Manuscript Accepted: 10 JUL 2013
- Harvard University CFAR. Grant Number: P30 AI060354
- NIH NEPRC. Grant Number: P51 OD011103
- innate immunity;
- lymphocyte homing;
- simian immunodeficiency virus
HIV and SIV infections induce NK cell dysfunction and hematopoietic defects in the bone marrow, but the effects of infection on bone marrow NK cell development and function are unknown.
Bone marrow NK cells were analyzed from both naïve and chronically SIV-infected rhesus macaques using polychromatic flow cytometry.
NK cell frequencies were reduced in infected compared with naïve animals, associated with increased apoptosis. Bone marrow NK cells from SIV-infected macaques upregulated perforin expression, suggesting increased cytotoxicity, and shifted toward a more mature CD16+ NK cell subpopulation phenotype. Unexpectedly, expression of the trafficking markers α4β7, CCR7, and CD62L was unchanged on bone marrow NK cells during SIV infection.
These data demonstrate that during SIV infection, bone marrow NK cells are reduced in number, but upregulate cytotoxic functions. Furthermore, our data suggest acquired cytotoxicity and loss may be due to in situ NK cell differentiation and not emigration.