Development of a novel rhesus macaque model with an infectious R5 simian–human immunodeficiency virus encoding HIV-1 CRF08_BC env

Authors

  • Li Huang,

    1. Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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  • Shigeru Kusagawa,

    1. Laboratory of Virology and Diagnosis, AIDS Research Center, National Institute of Infectious Disease, Toyama, Tokyo, Japan
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  • Haiyan Zeng,

    1. Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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  • Guibo Yang,

    1. National Center for AIDS/STD Control and Prevention, China Center for Disease Prevention and Control, Beijing, China
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  • Binlian Sun,

    1. Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
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  • Tomoyuki Miura,

    1. Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, Japan
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  • Rongge Yang

    Corresponding author
    1. Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
    • Correspondence

      Rongge Yang, Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Xiaohongshan 44, Wuhan 430071, China.

      Tel.: +86 27 87198736;

      fax: +86 27 87198736;

      e-mail: ryang@wh.iov.cn

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  • This work was performed at Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, The People's Republic of China.

Abstract

Background

The CRF08_BC strain is one of the most predominant circulating Human immunodeficiency virus type 1 (HIV-1) strains in the Chinese pandemic. A simian–human immunodeficiency virus (SHIV) encoding HIV-1 CRF08_BC env is highly desirable to evaluate candidate AIDS vaccines in non-human primates.

Methods

SHIV-KBQJ-12, which carries the envelope glycoprotein from QJ001, an infectious molecular clone of HIV-1 CRF08_BC, was generated. The replication capacity of SHIV-KBQJ-12 was determined both in human and rhesus macaque (Macaca mulatta) peripheral blood mononuclear cells (PBMCs) and in Chinese rhesus macaques.

Results

SHIV-KBQJ-12 replicated efficiently in human and macaque PBMCs and displayed a preference for CCR5 as an entry coreceptor. Productive infection of two macaques by intravenous inoculation with SHIV-KBQJ-12 was confirmed.

Conclusions

SHIV-KBQJ-12 is an R5-tropic chimeric virus that can establish productive infection both in vitro and in vivo in Chinese rhesus macaques and will be useful to assess candidate HIV-1 CRF08_BC vaccines in China.

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