These authors contributed equally to this work.
Neuronal Galectin-4 is required for axon growth and for the organization of axonal membrane L1 delivery and clustering
Version of Record online: 3 FEB 2013
© 2013 International Society for Neurochemistry
Journal of Neurochemistry
Volume 125, Issue 1, pages 49–62, April 2013
How to Cite
J. Neurochem. (2013) 125, 49–62.
- Issue online: 21 MAR 2013
- Version of Record online: 3 FEB 2013
- Accepted manuscript online: 12 JAN 2013 08:52PM EST
- Manuscript Accepted: 5 JAN 2013
- Manuscript Received: 20 DEC 2012
- EC Marie Curie RTN. Grant Number: 2005-019561
- EU 7th Framework Program. Grant Number: 26060
- Carlos III Health Institute and Castilla-La-Mancha Health Service (SESCAM) EMER program. Grant Number: EMER07/026
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Figure S1. (a) Gal-4 distribution in permeabilized and nonpermeabilized cortical neurons in culture, similar to hippocampal neurons shown in Fig. 1 (b) Gal-4 expression in both neuronal types analysed by Western blotting.
Figure S2. Control of secondary antibodies in Gal-4 immuno-histochemistry.
Figure S3. Sulfation inhibition does not modify intracellular Gal-4.
Figure S4. (a) Gal-4 segmented distribution in axons is not modied by lantrunculin-induced actin de-polymerization. (b) Gal-4 knockdown does not modify L1 biosynthesis.
Figure S5. Exogenous rGal4 is incorporated in neurons, traffics along the axon and reaches restricted zones of its membrane.
Figure S6. Proposed model for the axonal transport and clustering of L1, based on cross-linking of LacNAc termini in L1 N-glycans to sulfatide-containing vesicle carriers by the tandem-repeat-type Gal-4 with its two lectin sites.
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