Original Article

Insights into the oligomerization of CRMPs: crystal structure of human collapsin response mediator protein 5

  1. Rajesh Ponnusamy†,
  2. Bernhard Lohkamp*

Article first published online: 5 MAR 2013

DOI: 10.1111/jnc.12188

Additional Information

How to Cite

J. Neurochem.(2013)10.1111/jnc.12188

Author Information

  1. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

  1. †

    Division of Biological Chemistry, ITQB-Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal

*Address correspondence and reprint requests to Bernhard Lohkamp, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Tomtebodavägen 6, SE-17177 Stockholm, Sweden. E-mail: bernhard.lohkamp@ki.se

Publication History

  1. Article first published online: 5 MAR 2013
  2. Accepted manuscript online: 4 FEB 2013 07:00AM EST
  3. Manuscript Accepted: 7 JAN 2013
  4. Manuscript Revised: 19 DEC 2012
  5. Manuscript Received: 10 OCT 2012

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Figure S1. Sequence alignment of human CRMPs.

Figure S2. SDS-PAGE of CRMP-5 WT before and after crystallization.

Figure S3. Co-expression and purification of CRMP-1 with CRMP-2 and CRMP-5.

Figure S4. Ion location in CRMP-5 ΔC Zn soak.

Figure S5. Location of mutation sites in the arm-arm interface of CRMP-5 that affect CRMP function.

Table S1. Data collection and refinement statistics for CRMP-5 Zn soak

Table S2. Sequence identity of human CRMPs.

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