SEARCH

SEARCH BY CITATION

Keywords:

  • fluorescence imaging;
  • membrane dynamics;
  • STED nanoscopy

Abstract

Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics.