WNT-3A and WNT-5A counteract lipopolysaccharide-induced pro-inflammatory changes in mouse primary microglia
Article first published online: 22 APR 2013
© 2013 International Society for Neurochemistry
Journal of Neurochemistry
Volume 125, Issue 6, pages 803–808, June 2013
How to Cite
J. Neurochem.(2013) 125, 803–808.
- Issue published online: 5 JUN 2013
- Article first published online: 22 APR 2013
- Accepted manuscript online: 27 MAR 2013 09:31AM EST
- Manuscript Revised: 22 MAR 2013
- Manuscript Accepted: 22 MAR 2013
- Manuscript Received: 12 MAR 2013
Surveying microglia, the resident macrophage-like cells in the central nervous system, continuously screen their surroundings to sense imbalance in tissue homeostasis. Their activity is tightly regulated in both a pro- and anti-inflammatory manner. We have previously shown that the lipoglycoproteins WNT-3A and WNT-5A drive pro-inflammatory transformation in primary mouse microglia cells, arguing that WNTs have a role in the modulation of the central nervous system immune response. In this study, we address the effects of recombinant WNT-3A and WNT-5A on lipopolysaccharide (LPS)-activated mouse primary microglia to investigate the putative anti-inflammatory modulation of microglia by WNTs. While both WNT-3A and WNT-5A alone induce an up-regulation of cyclooxygenase 2 (COX2), a generic pro-inflammatory microglia marker, LPS exceeds these effects dramatically. However, combination of LPS and WNTs results in a dose-dependent decrease in LPS-induced cyclooxygenase 2 protein and mRNA expression. In conclusion, our data suggest that WNTs have a dual and context-dependent effect on microglia acting in a homeostatic pro- and anti-inflammatory manner.