Figure S1. Expression of α2AP in hippocampal neurons from WT mice. Primary hippocampal neurons from WT mice were immunostained with antibodies against α2AP and MAP2 at 2 DIV.

Figure S2. Reduction in the MAP2 expression and length of MAP2-positive neurites in hippocampal neurons from α2AP+/− mice compared with those in neurons from WT mice.

Figure S3. Distribution analyses of MAP2-positive neurite length and the number of tips in hippocampal neurons from α2AP−/− and WT mice.

Figure S4. α2AP-induced axonal elongation in hippocampal neurons. Primary hippocampal neurons from the WT and α2AP−/− mice were treated with α2AP (250 nM) at 1 DIV, and cultured for 24 h.

Figure S5. Little effect of α2AP on JNK and ERK activation in hippocampal neurons.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.