Distinct intrinsic and synaptic properties of pre-sympathetic and pre-parasympathetic output neurons in Barrington's nucleus

Authors

  • Yue-Xian Guo,

    1. Department of Anesthesiology and Perioperative Medicine, Center for Neuroscience and Pain Research, Houston, Texas, USA
    2. Department of Urology, The Third Hospital of HeBei Medical University, Shijiazhuang, HeBei, P.R. China
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    • These authors contributed equally to this study.
  • De-Pei Li,

    1. Department of Critical Care, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
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    • These authors contributed equally to this study.
  • Shao-Rui Chen,

    1. Department of Anesthesiology and Perioperative Medicine, Center for Neuroscience and Pain Research, Houston, Texas, USA
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  • Hui-Lin Pan

    Corresponding author
    • Department of Anesthesiology and Perioperative Medicine, Center for Neuroscience and Pain Research, Houston, Texas, USA
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Address correspondence and reprint requests to Dr. Hui-Lin Pan, Department of Anesthesiology and Perioperative Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Unit 110, Houston, TX 77030-4009, USA. E-mail: huilinpan@mdanderson.org

Abstract

Barrington's nucleus (BN), commonly known as the pontine micturition center, controls micturition and other visceral functions through projections to the spinal cord. In this study, we developed a rat brain slice preparation to determine the intrinsic and synaptic mechanisms regulating pre-sympathetic output (PSO) and pre-parasympathetic output (PPO) neurons in the BN using patch-clamp recordings. The PSO and PPO neurons were retrogradely labeled by injecting fluorescent tracers into the intermediolateral region of the spinal cord at T13-L1 and S1-S2 levels, respectively. There were significantly more PPO than PSO neurons within the BN. The basal activity and membrane potential were significantly lower in PPO than in PSO neurons, and A-type K+ currents were significantly larger in PPO than in PSO neurons. Blocking A-type K+ channels increased the excitability more in PPO than in PSO neurons. Stimulting μ-opioid receptors inhibited firing in both PPO and PSO neurons. The glutamatergic EPSC frequency was much lower, whereas the glycinergic IPSC frequency was much higher, in PPO than in PSO neurons. Although blocking GABAA receptors increased the excitability of both PSO and PPO neurons, blocking glycine receptors increased the firing activity of PPO neurons only. Furthermore, blocking ionotropic glutamate receptors decreased the excitability of PSO neurons but paradoxically increased the firing activity of PPO neurons by reducing glycinergic input. Our findings indicate that the membrane and synaptic properties of PSO and PPO neurons in the BN are distinctly different. This information improves our understanding of the neural circuitry and central mechanisms regulating the bladder and other visceral organs.

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