The pre-synaptic Munc13-1 binds alcohol and modulates alcohol self-administration in Drosophila
Article first published online: 10 JUN 2013
© 2013 International Society for Neurochemistry
Journal of Neurochemistry
Volume 126, Issue 6, pages 715–726, September 2013
How to Cite
J. Neurochem. (2013) 126, 715–726.
- Issue published online: 6 SEP 2013
- Article first published online: 10 JUN 2013
- Accepted manuscript online: 20 MAY 2013 11:37AM EST
- Manuscript Accepted: 17 MAY 2013
- Manuscript Revised: 9 MAY 2013
- Manuscript Received: 25 MAR 2013
- University of Houston. Grant Number: NIH 7R21AA016140
|jnc12315-sup-0001-SupplementalMaterials.docx||Word document||741K|| |
Data S1. Supplemental methods.
Figure S1. Characterization of Munc13-1 C1.
Figure S2. Circular dichroism spectra of Munc13-1 C1 and its mutants: Comparison of circular dichroism spectra of Munc13-1 C1 (____), E582A (………), E582L (__ __ __), and E582H (_ _ _).
Figure S3. 3-azibutanol and 3-azioctanol quench the fluorescence of Munc13-1C1.
Figure S4. Glu-582 as the site for 3-azibutanol.
Figure S5. Identification of Glu-599 as the site for 3-azibutanol in E582A.
Figure S6. Effect of alcohols on thermal unfolding of wild-type and mutant protein (E582A).
Table S1. Photolabeling of Munc13-1 C1 mutants analyzed by mass spectrometry.
Table S2. Summary of melting temperatures (Tm) of Munc13-1 C1 and its mutants determined by circular dichroism.
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