jnc12315-sup-0001-SupplementalMaterials.docxWord document741K

Data S1. Supplemental methods.

Figure S1. Characterization of Munc13-1 C1.

Figure S2. Circular dichroism spectra of Munc13-1 C1 and its mutants: Comparison of circular dichroism spectra of Munc13-1 C1 (____), E582A (………), E582L (__ __ __), and E582H (_ _ _).

Figure S3. 3-azibutanol and 3-azioctanol quench the fluorescence of Munc13-1C1.

Figure S4. Glu-582 as the site for 3-azibutanol.

Figure S5. Identification of Glu-599 as the site for 3-azibutanol in E582A.

Figure S6. Effect of alcohols on thermal unfolding of wild-type and mutant protein (E582A).

Table S1. Photolabeling of Munc13-1 C1 mutants analyzed by mass spectrometry.

Table S2. Summary of melting temperatures (Tm) of Munc13-1 C1 and its mutants determined by circular dichroism.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.