Figure S1. (a) Cultured hippocampal neurons expressing GFP were treated with vehicle or with 5 nm NRG1.

Figure S2. (a) High magnification images of dendrites of cultured cortical neurons generated from wild type (WT) or KALRN knockout (KO) mice, expressing GFP, with or without NRG1 treatment for 3-days.

Figure S3. hEK293 were mock transfected or transfected with erbB4 cDNA for 2-days.

Figure S4. NRG1 and erbB4 both increase spine size and knocking down kalirin blocks the ability of NRG1 and erbB4 to regulate spine size.

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