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Figure S1. Levels of iNOS, nNOS, Tau and pTau are unaltered in the brains of late middle aged eNOS-/- mice as compared with age-matched wild-type control animals.

Figure S2. Hippocampal levels of iNOS, nNOS, Tau, and pTau were not different between late middle aged eNOS-/- and late middle aged wild-type mice.

Figure S3. Levels of CD68, GFAP, and NeuN are unaltered in the hippocampus of late middle aged eNOS-/- mice.

Figure S4. APP and BACE1 protein levels were unaltered in brain microvessels of late middle aged eNOS-/- mice as compared with late middle aged wild-type control animals.

Figure S5. APP and BACE1 protein levels were unaltered in microvessels isolated from the hippocampus of late middle aged eNOS-/- mice as compared with late middle aged wild-type control animals.

Figure S6. No differences in locomotor activity was observed between late middle aged eNOS-/- and late middle aged wild-type mice.

Table S1. Peripheral parameters of 15-month-old wild type and eNOS-/- mice.

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