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Appendix S1. Supplementary materials and methods.

Figure S1. Original records of changes in electrochemical currents recorded by GLU (red) and GLU-null (blue) biosensors during a typical experiment with three repeated nicotine injections (bottom panel; injections are shown as arrows).

Figure S2. Mean (± SEM) changes in electrochemical currents recorded by GLU sensors in the NAcc after i.v. saline injection in awake, freely moving rats (n = 12).

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