Genomic editing opens new avenues for zebrafish as a model for neurodegeneration

Authors

  • Bettina Schmid,

    Corresponding author
    1. German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
    2. Munich Cluster for Systems Neurology (SyNergy), Munich, Germany
    • Address correspondence and reprint requests to Bettina Schmid, German Center for Neurodegenerative Diseases (DZNE), Schillerstr. 44, 80336 Munich, Germany. E-mail: bettina.schmid@dzne.lmu.de

    Search for more papers by this author
  • Christian Haass

    1. German Center for Neurodegenerative Diseases (DZNE), Munich, Germany
    2. Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians University Munich, Munich, Germany
    3. Munich Cluster for Systems Neurology (SyNergy), Munich, Germany
    Search for more papers by this author

Abstract

Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approaches.

Ancillary