Alanine-(87)-threonine polymorphism impairs signaling and internalization of the human P2Y11 receptor, when co-expressed with the P2Y1 receptor
Version of Record online: 13 FEB 2014
© 2014 International Society for Neurochemistry
Journal of Neurochemistry
Volume 129, Issue 4, pages 602–613, May 2014
How to Cite
J. Neurochem. (2014) 129, 602–613.
- Issue online: 23 APR 2014
- Version of Record online: 13 FEB 2014
- Accepted manuscript online: 29 JAN 2014 02:44AM EST
- Manuscript Accepted: 21 JAN 2014
- Manuscript Revised: 13 JAN 2014
- Manuscript Received: 17 OCT 2013
- Deutsche Forschungsgemeinschaft
Figure S1. Amplitudes of the [Ca2+]i rise after stimulation of HEK293 cells with (a) BzATP or 2-MeS-ADP and (b) with ATP alone or in combination with the P2Y1 receptor antagonist MRS2179.
Figure S2. Concentration–response curves for the rise of [Ca2+]i induced by BzATP, 2-MeS-ADP, and ATP. Data were obtained from HEK293 wild-type cells.
Figure S3. Amplitudes for the rise of [Ca2+]i induced by ATP, UTP, UDP, BzATP, and 2-MeS-ADP at a concentration of 10 µM each, in (a) HEK293 wild-type cells and (b) 1321N1 astrocytoma wild-type cells.
Figure S4. Co-expression of P2Y1-myc/His and P2Y11-GFP (upper row) or P2Y11A87T-GFP (lower row) in 1321N1 astrocytoma cells.
Figure S5. Fluorescence intensities of GFP and Alexa555 of 1321N1 astrocytoma cells co-expressing the P2Y1-myc/His and P2Y11-GFP or P2Y11A87T-GFP receptors.
Figure S6. Fluorescence intensities of HEK293 cells expressing either the P2Y11-GFP (n = 60), P2Y11A87T-GFP (n = 80), P2Y11A87S-GFP (n = 59), or P2Y11A87Y-GFP (n = 70) receptors.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.