Alanine-(87)-threonine polymorphism impairs signaling and internalization of the human P2Y11 receptor, when co-expressed with the P2Y1 receptor
Article first published online: 13 FEB 2014
© 2014 International Society for Neurochemistry
Journal of Neurochemistry
Volume 129, Issue 4, pages 602–613, May 2014
How to Cite
J. Neurochem. (2014) 129, 602–613.
- Issue published online: 23 APR 2014
- Article first published online: 13 FEB 2014
- Accepted manuscript online: 29 JAN 2014 02:44AM EST
- Manuscript Accepted: 21 JAN 2014
- Manuscript Revised: 13 JAN 2014
- Manuscript Received: 17 OCT 2013
- Deutsche Forschungsgemeinschaft
Figure S1. Amplitudes of the [Ca2+]i rise after stimulation of HEK293 cells with (a) BzATP or 2-MeS-ADP and (b) with ATP alone or in combination with the P2Y1 receptor antagonist MRS2179.
Figure S2. Concentration–response curves for the rise of [Ca2+]i induced by BzATP, 2-MeS-ADP, and ATP. Data were obtained from HEK293 wild-type cells.
Figure S3. Amplitudes for the rise of [Ca2+]i induced by ATP, UTP, UDP, BzATP, and 2-MeS-ADP at a concentration of 10 µM each, in (a) HEK293 wild-type cells and (b) 1321N1 astrocytoma wild-type cells.
Figure S4. Co-expression of P2Y1-myc/His and P2Y11-GFP (upper row) or P2Y11A87T-GFP (lower row) in 1321N1 astrocytoma cells.
Figure S5. Fluorescence intensities of GFP and Alexa555 of 1321N1 astrocytoma cells co-expressing the P2Y1-myc/His and P2Y11-GFP or P2Y11A87T-GFP receptors.
Figure S6. Fluorescence intensities of HEK293 cells expressing either the P2Y11-GFP (n = 60), P2Y11A87T-GFP (n = 80), P2Y11A87S-GFP (n = 59), or P2Y11A87Y-GFP (n = 70) receptors.
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