jnc12666-sup-0001-FigS1-S6.docWord document678K

Figure S1. Amplitudes of the [Ca2+]i rise after stimulation of HEK293 cells with (a) BzATP or 2-MeS-ADP and (b) with ATP alone or in combination with the P2Y1 receptor antagonist MRS2179.

Figure S2. Concentration–response curves for the rise of [Ca2+]i induced by BzATP, 2-MeS-ADP, and ATP. Data were obtained from HEK293 wild-type cells.

Figure S3. Amplitudes for the rise of [Ca2+]i induced by ATP, UTP, UDP, BzATP, and 2-MeS-ADP at a concentration of 10 µM each, in (a) HEK293 wild-type cells and (b) 1321N1 astrocytoma wild-type cells.

Figure S4. Co-expression of P2Y1-myc/His and P2Y11-GFP (upper row) or P2Y11A87T-GFP (lower row) in 1321N1 astrocytoma cells.

Figure S5. Fluorescence intensities of GFP and Alexa555 of 1321N1 astrocytoma cells co-expressing the P2Y1-myc/His and P2Y11-GFP or P2Y11A87T-GFP receptors.

Figure S6. Fluorescence intensities of HEK293 cells expressing either the P2Y11-GFP (n = 60), P2Y11A87T-GFP (n = 80), P2Y11A87S-GFP (n = 59), or P2Y11A87Y-GFP (n = 70) receptors.

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