Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats

Authors

  • Gustavo O. Puntel,

    1. Departamento de Morfologia, Centro de Ciências de Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
    2. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • Nélson R. Carvalho,

    1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • Fernando Dobrachinski,

    1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • Andréia C. F. Salgueiro,

    1. Universidade Federal do Pampa, UNIPAMPA – Campus Uruguaiana, Uruguaiana, RS, Brasil
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  • Robson L. Puntel,

    1. Universidade Federal do Pampa, UNIPAMPA – Campus Uruguaiana, Uruguaiana, RS, Brasil
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  • Vanderlei Folmer,

    1. Universidade Federal do Pampa, UNIPAMPA – Campus Uruguaiana, Uruguaiana, RS, Brasil
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  • Nilda B. V. Barbosa,

    1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • Luiz F. F. Royes,

    1. Departamento de Métodos e Técnicas Desportivas, Centro de Educação Física e Desportos, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • João Batista T. Rocha,

    1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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  • Félix A. A. Soares

    Corresponding author
    1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
    • Departamento de Morfologia, Centro de Ciências de Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
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Correspondence

Félix Alexandre Antunes Soares, Departamento de Química, CCNE, Universidade Federal de Santa Maria, 97105-900 – Santa Maria, RS, Brazil. T: +55 XX 55 3220 9522; F: +55 XX 55 3220 8978; E: felix_antunes_soares@yahoo.com.br

Abstract

The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused (I/R) gastrocnemius muscle of rats. Forty male Wistar rats were divided into control and I/R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid-reactive substances and dichlorofluorescein oxidation levels were observed in I/R muscle. Cryotherapy in I/R muscle also minimized effects such as decreased cellular viability, levels of non-protein thiols and calcium ATPase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I/R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I/R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction.

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