Topically delivered dissolved oxygen reduces inflammation and positively influences structural proteins in healthy intact human skin
Article first published online: 3 JUN 2013
© 2013 Wiley Periodicals, Inc.
Journal of Cosmetic Dermatology
Volume 12, Issue 2, pages 86–95, June 2013
How to Cite
Kellar, R. S., Audet, R. G., Roe, D. F., Rheins, L. A. and Draelos, Z. D. (2013), Topically delivered dissolved oxygen reduces inflammation and positively influences structural proteins in healthy intact human skin. Journal of Cosmetic Dermatology, 12: 86–95. doi: 10.1111/jocd.12039
- Issue published online: 3 JUN 2013
- Article first published online: 3 JUN 2013
- Manuscript Accepted: 15 JAN 2013
- topically dissolved oxygen;
- structural proteins;
- aquaglyceroporin channel;
As oxygen is essential for wound healing and there is limited diffusion across the stratum corneum into the epidermis, we wanted to evaluate whether the topical delivery of a total dissolved oxygen in dressing form on intact human subject skin would improve clinical and histologic skin functioning.
Fifty normal, healthy subjects completed a pilot clinical evaluation to assess the efficacy and tolerability of a dissolved oxygen dressing (OxygeneSys™-Continuous) to improve the health and appearance of intact skin.
Clinical analysis was performed on 50 subjects; histological and gene expression analysis was performed on 12 of the 50 subjects to assess the effect of the dissolved oxygen dressing.
Clinical data demonstrate that the dressing is well tolerated, and several measures of skin health and integrity showed improvements compared with a control dressing site. Skin hydration measurements showed a statistically significant increase in skin hydration at 0–4, 4–8, and 0–8 weeks (P < 0.05 at each time point). The blinded clinical investigator's grading of desquamation, roughness, and skin texture show significant decreases from baseline to the 8-week time point (P < 0.05). The dressings were removed prior to the blinded clinical investigator's grading. These data were supported by the histological and gene expression studies, which showed a general reduction in inflammatory response markers and transcription products (IL-6, IL-8, TNF-alpha, MMP-1, and MMP-12), while facilitating a general increase in structural skin proteins (collagen I, elastin, and filaggrin). Additionally, p53 signals from biopsy samples support the clinical investigator's observations of no safety concerns.
The data from this study demonstrate that the dressing has no deleterious effects and stimulates beneficial effects on intact, nonwounded skin.