MicroRNA-137 promoter methylation in oral lichen planus and oral squamous cell carcinoma

Authors

  • Jun Dang,

    1. Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
    2. Department of Stomatology, Affiliated Hospital of the Academy of Military Medical Sciences, Beijing, China
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  • Yong-Qian Bian,

    1. Department of General Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China
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  • Jian Yong Sun,

    1. Department of General Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China
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  • Fang Chen,

    1. Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Guang-Ying Dong,

    1. Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Qing Liu,

    1. Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Xin-Wen Wang,

    1. Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Jørgen Kjems,

    1. The Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
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  • Shan Gao,

    Corresponding author
    1. The Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
    • Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Qin-Tao Wang

    Corresponding author
    • Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an, China
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Correspondence: Qin-Tao Wang, DDS, Department of Periodontics and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Tel: 86 29 84776096, Fax: 86 29 83223047,E-mail: qintaowang@hotmail.com

Shan Gao, PhD, The Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University, C.F. Møllers Alle, Building 1130, 8000 Aarhus C, Denmark. Tel: 0045 87154975, Fax: 0045 86196500, E-mail: shg@mb.au.dk

Abstract

Oral lichen planus (OLP) is a common oral mucosal disease, which is generally considered a potentially malignant lesion. To identify efficiently prognostic biomarker, we investigated the microRNA-137 (miR-137) promoter methylation in OLP and compared with the samples from healthy volunteers and patients with oral squamous cell carcinoma (OSCC). A total of 20 OLP and 12 patients with OSCC as well as 10 healthy subjects were subjected to miR-137 promoter methylation analysis using methylation-specific PCR (MSP). To address the malignancy prediction potential from miR-137 promoter methylation status, methylation of the p16 gene, a well-known tumor suppressor, was investigated in the same samples. The p16 methylation and miR-137 promoter methylation were found to be 25% and 35% in patients with OLP, 50% and 58.3% in patients with OSCC, and 0% and 0% in healthy subjects, respectively. The differences between miR-137 and p16 methylation levels were statistically significant between healthy controls and patients. Methylation levels of the two promoters were also influenced by age, gender, and lesion duration. Interestingly, aberrant promoter methylation of the p16 and miR-137 genes was only found in the epithelium but not in the connective tissue from patients with OLP. This raises the possibility to use miR-137 methylation as a biomarker for malignant prediction in patients with OLP.

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