Delayed progression of condylar cartilage degeneration, by reduction of the discoidin domain receptor 2, in the temporomandibular joints of osteoarthritic mouse models

Authors

  • Armando Salazar,

    1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA
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    • These authors contribute equally to this work.
  • Ilona Polur,

    1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA
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    • These authors contribute equally to this work.
  • Jacqueline M. Servais,

    1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA
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  • Yefu Li,

    1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA
    2. Faculty of Medicine, Harvard Medical School, Boston, MA, USA
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  • Lin Xu

    Corresponding author
    1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA
    2. Faculty of Medicine, Harvard Medical School, Boston, MA, USA
    • Correspondence: Lin Xu, MD, PhD, REB. Rm514, 188 Longwood Ave. Boston, MA 02115, USA. Tel: 617 432 1764, Fax: 617 432 3246, E-mail: lin_xu@hms.harvard.edu

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Abstract

Objective

To determine whether reduction of the discoidin domain receptor 2 (Ddr2) delays the progression of condylar cartilage degeneration in the temporomandibular joint (TMJ) of mouse models with osteoarthritis (OA).

Methods

Double-heterozygous (Col11a1- and Ddr2-haploinsufficiency, Col11a1+/−;Ddr2+/−) mice were generated. TMJs of Ddr2+/− mice were subjected to partial discectomy. Condylar cartilage from the TMJ of Col11a1+/−;Ddr2+/− mice, surgically treated (discectomy) Ddr2+/− mice, and their corresponding controls was characterized by means of histology and evaluated using a scoring system specific to mouse joints.

Results

The progression of condylar cartilage degeneration was significantly delayed in the TMJ of Col11a1+/−;Ddr2+/− mice compared with those of the Col11a1+/− mice. The progression of condylar cartilage degeneration in the TMJ of Ddr2+/− mice following discectomy was also significantly delayed when compared with their wild-type littermates.

Conclusion

Reduced expression of Ddr2 delays the progression of condylar cartilage degeneration, induced either by type XI collagen haploinsufficiency or by a partial discectomy, in TMJ.

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