Anti-inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharide-stimulated BV-2 microglial cells
Article first published online: 18 MAR 2013
© 2013 Royal Pharmaceutical Society
Journal of Pharmacy and Pharmacology
Volume 65, Issue 6, pages 895–906, June 2013
How to Cite
Kim, S., Kim, J.-I., Choi, J.-W., Kim, M., Yoon, N. Y., Choi, C.-G., Choi, J.-S. and Kim, H.-R. (2013), Anti-inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharide-stimulated BV-2 microglial cells. Journal of Pharmacy and Pharmacology, 65: 895–906. doi: 10.1111/jphp.12049
- Issue published online: 5 MAY 2013
- Article first published online: 18 MAR 2013
- Manuscript Accepted: 15 JAN 2013
- Manuscript Received: 21 JUN 2012
- National Fisheries Research & Development Institute. Grant Number: RP-2012-BT
- Brain Busan 21, Busan metropolitan city, Republic of Korea
- Myagropsis myagroides;
- nuclear factor-κB
Microglial activation has been implicated in neurological disorders for its inflammatory and neurotrophic effects. We investigated the anti-inflammatory effect of the hexane fraction from Myagropsis myagroides (Mertens ex Turner) Fensholt ethanolic extract and its underlying molecular mechanism in lipopolysaccharide-stimulated microglia.
Various solvent fractions prepared from the ethanolic extract of M. myagroides were analysed for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity and inhibitory effect on nitric oxide (NO) production in activated BV-2 microglia. We measured prostaglandin E2 (PGE2) and pro-inflammatory cytokine levels by enzyme-linked immunosorbent assay. Expression of inflammatory enzymes was analysed by Western blot. Nuclear translocation and activation of nuclear factor-kappaB (NF-κB) were determined by immunofluorescence and reporter gene assay, respectively.
Among the fractions, the hexane fraction (MMH), rich in fatty acid, showed the highest inhibitory activity on NO generation. Pretreatment with MMH decreased mRNA and protein levels of inducible NO synthase and cyclooxygenase-2, resulting in a decrease in NO and PGE2 in LPS-stimulated BV-2 cells. Furthermore, MMH inhibited the production of inducible pro-inflammatory cytokines at their transcriptional level via inactivation of NF-κB. MMH inhibited the activation of extracellular signal-regulated kinase and c-Jun N-terminal kinase.
These results indicate that MMH has a strong anti-inflammatory activity in LPS-stimulated microglia, suggesting that MMH can be used as a therapeutic agent against neuroinflammatory diseases.