Tupistra chinensis extract attenuates murine fulminant hepatitis with multiple targets against activated T lymphocytes
Version of Record online: 10 NOV 2013
© 2013 Royal Pharmaceutical Society
Journal of Pharmacy and Pharmacology
Volume 66, Issue 3, pages 453–465, March 2014
How to Cite
Wu, X., Fan, J., Ouyang, Z., Ning, R., Guo, W., Shen, Y., Wu, X., Sun, Y. and Xu, Q. (2014), Tupistra chinensis extract attenuates murine fulminant hepatitis with multiple targets against activated T lymphocytes. Journal of Pharmacy and Pharmacology, 66: 453–465. doi: 10.1111/jphp.12176
- Issue online: 17 FEB 2014
- Version of Record online: 10 NOV 2013
- Manuscript Accepted: 10 OCT 2013
- Manuscript Received: 6 MAY 2013
- National Natural Science Foundation of China. Grant Numbers: 81173070, 81001465
- Natural Science Foundation of Jiangsu Province. Grant Numbers: BK2011053, BK20131282
- National Science & Technology Major Project. Grant Number: 2012ZX09304-001
- Con A;
- experimental hepatitis;
- T cells;
- Tupistra chinensis
The extract of Tupistra chinensis (TCE) is traditionally used for the treatment of inflammatory diseases in southwestern China for hundreds of years. The present study was designed to investigate the effects of the TCE against experimental hepatitis and to illustrate its potential mechanisms.
Effects of TCE were investigated on Con A-induced hepatitis. Profiles of multiple cytokines were measured with biometric immuno-sandwich ELISA. Proliferation, activation and apoptosis of T lymphocytes were evaluated using Western blot, MTT analysis and flow cytometry.
TCE significantly inhibited levels of serum transaminases and lactic dehydrogenase in mice with Con A-induced hepatitis, accompanied with marked alleviation of the liver microscopic appearances. Moreover, it decreased levels of inflammatory cytokines in a concentration-dependent manner both in vivo and in vitro. It also suppressed mitogen-activated protein kinases and NF-κB-signalling in liver. These effects of TCE are attributed to its inhibition on activated T cells but not to hepatocytes protection. Flow cytometry and immunoblot assay data showed its effects on STAT1/NF-κB-signalling blockage and apoptosis induction in activated T cells.
Our findings illustrate the significant potential of TCE as a novel approach for treatment of T cell-mediated inflammatory diseases.