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jpy12091-sup-0001-FigureS1.tifimage/tif679KFigure S1. Pictorial representation of the micropicking process using a specially designed syringe to pick a non-contaminated colony.
jpy12091-sup-0002-FigureS2.tifimage/tif1461KFigure S2. Microalgal strains grown under different trophic states in liquid media containing glucose (10,000 ppm) to confirm axenicity. CV: Chlorella vulgaris; CS: Chlorella sorokiniana; Sc: Scenedesmus sp. YC001; L: Light; D: Dark; PC1: Sorted Chlorella cells; PC2: Enriched Chlorella culture; PC3: Sorted Scenedesmus cells; PC4: Ultrasonicated Scenedesmus culture; NC1,2: No inoculant.
jpy12091-sup-0003-FigureS3.tifimage/tif4109KFigure S3. DAPI staining followed by fluorescence microscopy confirms axenicity of Chlorella vulgaris cells (A) and Scenedesmus sp. YC001 cells (C), and reveals contamination in enriched cultures of C. vulgaris (B) and Scenedesmus sp. YC001 (D) – blue spots indicating bacterial DNA.
jpy12091-sup-0004-TableS1.docWord document67KTable S1. Sequence identities of DGGE bands and those of closest relatives.

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