jpy12096-sup-0001-FigureS1.pdfapplication/PDF175KFigure S1. CLUSTAL 2.1 multiple sequence alignment of α-tubulin sequences from a Fucus serrtus EST data set (Pearson et al. 2010). The alignment shows the region of TC_00471 and TC_00024 between the MB1 and MB2 primer binding sites (indicated by ‘+’ above the sequence in the 5′ and 3′ regions of TC_00471). In the predicted protein coding sequence of TC_00471 and TC_00024 there are 15 nucleotide substitutions in 333 bases from the MB1 primer binding site to the predicted stop codon (Indicated by ‘XXX’). The region spanning the stop codon to the MB2 primer binding site in TC_00471 is less well conserved and identity is low at the MB2 primer binding site.
jpy12096-sup-0002-FigureS2.pdfapplication/PDF200KFigure S2. Sequence of the Fucus serratus β-actin mRNA that was used to construct templates for the generation of dsRNA in this work. The sequence includes the 69 nucelotide 5′UTR region (underlined), the complete coding sequence delineated by ATG (Start) and TAA (Stop) codons shown in capitalized text and the 157 nucleotide 3′UTR region (underlined). The binding sites of forward (f) and reverse (r) primers used to generate the template sequences for the 551 bp (Actsf1 and Actfs2) and 1200 bp (MB3 and MB4) dsRNA actin constructs are shown in bold text.
jpy12096-sup-0003-FigureS3.tifimage/tif9251KFigure S3. Labeling of α-tubulin in putative polyspermic Fucus serratus zygotes. (a) Brightfield image of a polyspermic embryo. (b, c) Representative α-tubulin labeling in similar zygotes.

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