jpy12099-sup-0001-FigureS1.tifimage/tif826KFigure S1. Relationship between ocean temperature and dissolved inorganic nitrogen concentration (DIN) (NO2 + NO3) for Stillwater Cove, Pebble Beach, California. Temperature data were obtained from an array of four Onset StowAway Tidbits (±0.2°C) positioned at 0.5, 4, 8, and 13.5 m along a mooring line at the study site. Nutrient data were obtained from 50 discrete water samples collected throughout the water column, adjacent to the temperature loggers between 2010 and 2012. Water samples were frozen after collection and analyzed at the Oceanic Nutrient Laboratory at the University of South Florida. The line represents best fit (Least-squares linear regression: DIN = 59.68 (±2.94) + −3.75 (±0.25) * temperature; F(1,48) = 216.346, P ≪ 0.001, r2 = 0.818).
jpy12099-sup-0002-FigureS2.tifimage/tif841KFigure S2. Time series from the Stillwater Cove mooring from July 13 to August 22, 2012. (a) Daily mean water column temperature derived from the measurements collected at five minute intervals from 0.5, 4, 8, and 13.5 m depth, (b) daily mean (NO2 + NO3) concentration calculated using the linear relationship presented in Fig. S1.
jpy12099-sup-0003-FigureS3.tifimage/tif823KFigure S3. Frond biomass as a function of frond length determined from 39 individual fronds collected from adult Macrocystis sporophytes at the study site between March and August 2012. Intact fronds were collected by divers from randomly selected sporophytes, rehydrated to a constant weight at the lab, and wet weight was determined to the nearest 0.01 kg. The equation derived from the quadratic fit was used to calculate biomass in this experiment using a site-specific mean frond length calculated from 85 randomly selected fronds >1 m between 2010 and 2012. The line represents best fit (Least-squares polynomial regression: Biomass = 0.182 (±0.123) − 0.01 (±0.044) * frond length + 0.017 (±0.003) * frond length2; F(2,36) = 232.119, P = ≪ 0.001, r2 = 0.924).
jpy12099-sup-0004-TableS1.pdfapplication/PDF214KTable S1. Summary statistics for ANCOVAs run against all five examined chemical variables. Degrees of freedom are reported in parentheses for all dates for δ13C and are consistent for all other variables tested. For all factors except tissue, deviations from 21 degrees of freedom are due to sample loss. Bold values indicated P < 0.05.

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