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jpy12128-sup-0001-FigS1.tifimage/tif19348KFigure S1. Morphological diversity within Microcoleus Clades B–F (Fig. 2). Strains are presented in same order as in Figure 2 (top to bottom of phylogeny) and strain numbers are embedded in the figure. (A–C) Clade B. (D–G) Clade C. (H) M. autumnalis Luznice. (I) Microcoleus Kralovice. (J–M) Clade D. (N–P) Clade E. (Q and R) Clade F. All figures are to the same magnification, scale represents 20 μm.
jpy12128-sup-0002-FigS2.tifimage/tif906KFigure S2. Phylogenetic relationships within the Oscillatoriales, showing the relationship of Microcoleus sensu stricto within the order Oscillatoriales. Maximum likelihood method based on the Tamura three-parameter model in MEGA 5 was employed. Topology was validated by unweighted maximum parsimony in PAUP*. Numbers indicate bootstrap values for ML and MP (1,000 replicates) and bayesian posterior probabilities. Asterisk (*) indicates a value 100 for ML and MP or Bayesian posterior probability of 1.00. Values lower than 50% are not shown. A fragment of 1109 nt was used for the phylogenetic analysis of the 16S rRNA gene.
jpy12128-sup-0003-TableS1.docxWord document18KTable S1. Geographic origin, collection habitat, and year of collection for all strains sequenced by us (taxon name adopted from current work, not historical designation at time of isolation). Strains are arranged in the order they appear in Figure 5 (top to bottom).
jpy12128-sup-0004-TableS2.docxWord document16KTable S2. Percent similarity matrix (P-distance) of partial 16S rRNA gene sequence (1,072 bp compared) for Microcoleus vaginatus SAG 22.11 and selected oscillatorialean strains outside of the Microcoleus sensu stricto clade. Only the species pairs Phormidium animale CCALA761: Geitlerinema sp. CCALA138 and P. pseudopristleyi ACEV53: P. animale CCALA140 could reliably be placed in the same genera (and for both pairs, possibly the same species), demonstrating the error-prone taxonomy reported for cyanobacteria in GenBank.
jpy12128-sup-0005-TableS3.docxWord document22KTable S3. Cellular dimensions for a sample of Microcoleus species, arranged in same order (top to bottom) as in Figure 5, clades (Cl.) represent putative species (Fig. 5).

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