Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino-acid profiling and in vivo analysis
Article first published online: 4 AUG 2014
© 2014 Phycological Society of America
Journal of Phycology
Volume 50, Issue 5, pages 837–849, October 2014
How to Cite
Willis, A., Eason-Hubbard, M., Hodson, O., Maheswari, U., Bowler, C., Wetherbee, R. (2014), Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino-acid profiling and in vivo analysis. Journal of Phycology, 50: 837–849. doi: 10.1111/jpy.12214
- Issue published online: 1 OCT 2014
- Article first published online: 4 AUG 2014
- Accepted manuscript online: 5 JUL 2014 08:36AM EST
- Manuscript Accepted: 22 MAY 2014
- Manuscript Received: 17 DEC 2012
- Akzo Nobel - International Paints and the Defence Science and Technology Organisation
- Agence Nationale de Recherche
- European Union
Figure S1. Confocal microscopy images of PDC2:YFP localization at different times during cell division. Scanning confocal light microscope images of the PDC2:YFP gene product in transgenic cells, as depicted by YFP signal (column a); yellow fluorescence is YFP, the red signal is chlorophyll autofluorescence; and transmission images of corresponding cells under white light (column b).
Table S1. The top 37 putative P. tricornutum CAM genes, with manual annotation. The first ten are the “Top Ten” selected genes. (Protein IDs are as in the JGI portal, http://genome.jgi-psf.org/Phatr2/Phatr2.home.html).
Table S2. List of primers for “Top Ten” genes and YFP, as used for PCR screening. Protein IDs in the JGI portal are indicated (http://genome.jgi-psf.org/Phatr2/Phatr2.home.html) together with list of pDEST vectors used and the pEXP vectors created for transformation of P. tricornutum cells.
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