Phylogeny of North American Tolypella (Charophyceae, Charophyta) based on plastid DNA sequences with a description of Tolypella ramosissima sp. nov.
Version of Record online: 16 AUG 2014
© 2014 Phycological Society of America
Journal of Phycology
Volume 50, Issue 5, pages 776–789, October 2014
How to Cite
Pérez, W., Hall, J. D., McCourt, R. M., Karol, K. G. (2014), Phylogeny of North American Tolypella (Charophyceae, Charophyta) based on plastid DNA sequences with a description of Tolypella ramosissima sp. nov. Journal of Phycology, 50: 776–789. doi: 10.1111/jpy.12219
- Issue online: 1 OCT 2014
- Version of Record online: 16 AUG 2014
- Accepted manuscript online: 14 JUL 2014 11:54PM EST
- Manuscript Accepted: 16 MAY 2014
- Manuscript Received: 7 NOV 2013
- National Science Foundation. Grant Numbers: DEB-1020660, DEB-1020948
- American Society of Plant Taxonomists
- City University of New York
- Phycological Society of America
- Cullman Program for Molecular Systematics of The New York Botanical Garden
- atpB, psbC, and rbcL phylogeny;
- North America;
- Tolypella ;
- Tolypella ramosissima sp. nov.
Characeae (Charophyceae, Charophyta) contains two tribes with six genera: tribe Chareae with four genera and tribe Nitelleae, which includes Tolypella and Nitella. This paper uses molecular and morphological data to elucidate the phylogeny of Tolypella species in North America. In the most comprehensive taxonomic treatment of Characeae, 16 Tolypella species worldwide were subsumed into two species, T. intricata and T. nidifica, in two sections, Rothia and Tolypella respectively. It was further suggested that Tolypella might be a derived group within Nitella. In this investigation into species diversity and relationships in North American Tolypella, sequence data from the plastid genes atpB, psbC, and rbcL were assembled for a broad range of charophycean and land plant taxa. Molecular data were used in conjunction with morphology to test monophyly of the genus and species within it. Phylogenetic analyses of the sequence data showed that Characeae is monophyletic but that Nitelleae is paraphyletic with Tolypella sister to a monophyletic Nitella + Chareae. The results also supported the monophyly of Tolypella and the sections Rothia and Tolypella. Morphologically defined species were supported as clades with little or no DNA sequence differences. In addition, molecular data revealed several lineages and a new species (T. ramosissima sp. nov.), which suggests greater species diversity in Tolypella than previously recognized.