Quality of canine spermatozoa retrieved by percutaneous epididymal sperm aspiration
Article first published online: 1 FEB 2013
© 2013 British Small Animal Veterinary Association
Journal of Small Animal Practice
Volume 54, Issue 2, pages 87–91, February 2013
How to Cite
Varesi, S., Vernocchi, V., Faustini, M. and Luvoni, G.C. (2013), Quality of canine spermatozoa retrieved by percutaneous epididymal sperm aspiration. Journal of Small Animal Practice, 54: 87–91. doi: 10.1111/jsap.12020
- Issue published online: 1 FEB 2013
- Article first published online: 1 FEB 2013
- Accepted: 7 December 2012
To investigate the feasibility of percutaneous epididymal sperm aspiration in dogs and whether it might provide a population of epididymal spermatozoa similar to the population that can be obtained by processing isolated epididymis caudae.
Concentration and total sperm number, motility, morphology and acrosomal integrity of spermatozoa retrieved by percutaneous epididymal sperm aspiration, in vitro aspiration and mincing of the cauda of the epididymis were compared.
Percutaneous epididymal sperm aspiration is a feasible procedure to retrieve a population of spermatozoa in dogs. Quality is similar to that of spermatozoa collected in vitro, although a wide variation amongst animals was observed.
In case of ejaculation failure due to pathological conditions in dogs, the collection of spermatozoa from the cauda of the epididymis could be an option for providing gametes for assisted reproductive technologies. Percutaneous epididymal sperm aspiration can be used in dogs with compromised reproductive performance, in which orchiectomy cannot be performed for medical or owner reasons. Further studies aimed to investigate whether the percutaneous epididymal sperm aspiration technique might be feasible for repeated semen collection and to accurately evaluate side effects are required.