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Fig. S1. Effects of thrombin or collagen stimulation on apoptosis induced by ABT-737 in human platelets. Human washed platelets were pretreated with thrombin (0.1 U mL−1, 1U mL−1) or collagen (0.5 μg mL−1, 2 μg mL−1) for 10 min, then incubated with ABT-737 (10 μM) for 3 h. The typical apoptotic events in platelets were determined including: (A) PS exposure, (B) ΔΨm depolarization and (C) caspase-3 activity. Data are represented as Means ± SEM from 3 - 4 independent experiments.

Fig. S2. Protective effects of P2Y12 activation on apoptosis induced by ABT-737 in mouse platelets. Mouse washed platelets were pretreated with PIK3 inhibitor wortmannin (100 nM), LY294002 (25 uM) or P2Y12 agonist AR-C69931MX (ARC, 100 nM), for 10 min, then incubated with ABT-737 (10 μM) for 3 h in the absence or presence of P2Y12 activation. The typical apoptotic events in platelets were determined including: (A) PS exposure, (B) ΔΨm depolarization and (C) caspase-3 activity. Data are represented as Means ± SEM from 3 - 4 independent experiments. *P < 0.05 and **P < 0.01 compared with control group; #P < 0.05 and ##P < 0.01 compared with the ABT-737 + MRS2179 + ADP treated group.

Fig. S3. P2Y1 activation alone induces PS exposure in human platelets. Human washed platelets were pretreated with P2Y12 receptor antagonist AR-C69931MX (ARC, 100 nM) or vehicle, then was stimulated with ADP (5 μM) for 10 min. Alternatively, human washed platelets were treated with serotonin (50 μM) for 5 min. PS exposure was determined by flow cytometry. Means ± SEM of PS exposure are shown (n = 3). **P < 0.01 compared with ADP + AR-C69931MX group.

FilenameFormatSizeDescription
JTH_12063_sm_FigS1.tif411KSupporting info item
JTH_12063_sm_FigS2.tif1704KSupporting info item
JTH_12063_sm_FigS3.tif610KSupporting info item

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