Biochemical and functional characterization of a recombinant monomeric factor VIII–Fc fusion protein
Version of Record online: 27 JAN 2013
© 2012 International Society on Thrombosis and Haemostasis
Journal of Thrombosis and Haemostasis
Volume 11, Issue 1, pages 132–141, January 2013
How to Cite
PETERS, R. T., TOBY, G., LU, Q., LIU, T., KULMAN, J. D., LOW, S. C., BITONTI, A. J. and PIERCE, G. F. (2013), Biochemical and functional characterization of a recombinant monomeric factor VIII–Fc fusion protein. Journal of Thrombosis and Haemostasis, 11: 132–141. doi: 10.1111/jth.12076
- Issue online: 27 JAN 2013
- Version of Record online: 27 JAN 2013
- Accepted manuscript online: 4 DEC 2012 03:15AM EST
- Received 18 May 2012, accepted 6 November 2012
Figure S1. Amino acid sequence of rFVIIIFc.
Figure S2. Thrombin mapping of rFVIIIFc and rBDD FVIII by liquid chromatography/mass spectrometry: Deconvoluted mass spectra of the Fc fragment of rFVIIIFc (A), and the spectra for the 6 kDa light-chain N-terminus peptide fragment of rFVIIIFc (B and C) and rBDD FVIII (D and E).
Figure S3. Surface plasmon resonance sensorgrams that depict the interactions of (A) rFVIIIFc and (B) rBDD FVIII with immobilized hVWF.
Table S1. Identification of Fc containing different N-linked glycans of rFVIIIFc.
Table S2. Identification of 6 kDa LC N-terminus peptide of rFVIIIFc.
Table S3. Identification of heterogenities of A2 domain of rFVIIIFc.
Table S4. List of the major peptides of rFVIIIFc after LysC digest and MS analysis.
|JTH_12076_sm_FigS1-S3-TableS1-S4.pdf||604K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.