Both authors equally contributed to this manuscript.
Innovation in detection of microparticles and exosomes
Article first published online: 30 JUN 2013
© 2013 International Society on Thrombosis and Haemostasis
Journal of Thrombosis and Haemostasis
Special Issue: State of the Art 2013
Volume 11, Issue Supplement s1, pages 36–45, June 2013
How to Cite
Innovation in detection of microparticles and exosomes. J Thromb Haemost 2013; 11 (Suppl. 1): 36–45., , , , .
- Issue published online: 30 JUN 2013
- Article first published online: 30 JUN 2013
- flow cytometry;
- raman spectroscopy;
- secretory vesicles
Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as blood. Although such vesicles have gained strong clinical and scientific interest, their detection is difficult because many vesicles are extremely small with a diameter of less than 100 nm, and, moreover, these vesicles have a low refractive index and are heterogeneous in both size and composition. In this review, we focus on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and we will discuss their applicability and limitations. Finally, we discuss four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small-angle X-ray scattering (SAXS), and anomalous SAXS. These methods are currently being explored to study vesicles and are likely to offer novel information for future developments.