These authors contributed equally to this work.
Quantification of hepatitis B surface antigen and E antigen: correlation between Elecsys and Architect assays
Article first published online: 25 MAR 2013
© 2013 John Wiley & Sons Ltd
Journal of Viral Hepatitis
Volume 20, Issue 6, pages 422–429, June 2013
How to Cite
Zhou, B., Liu, M., Lv, G., Zheng, H., Wang, Y., Sun, J. and Hou, J. (2013), Quantification of hepatitis B surface antigen and E antigen: correlation between Elecsys and Architect assays. Journal of Viral Hepatitis, 20: 422–429. doi: 10.1111/jvh.12044
- Issue published online: 7 MAY 2013
- Article first published online: 25 MAR 2013
- Manuscript Accepted: 29 OCT 2012
- Manuscript Received: 16 AUG 2012
- National twelve-five project of China . Grant Number: 2012ZX10002-004
- hepatitis B e antigen;
- hepatitis B surface antigen;
Quantification of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) and their change model during treatment are emerging as a useful tool for assessing the outcome of hepatitis B virus (HBV) infection and predicting the efficacy of antiviral therapy. The aim of this study was to compare the performance of the Elecsys and Architect assays for HBsAg and HBeAg quantification. Quantification of HBsAg and HBeAg, determined by these two assays, were assessed in 1292 sera from patients with chronic hepatitis B(CHB). HBeAg quantification in serum was performed by calibrating the results through HBeAg Paul-Ehrlich international (PEI) reference standard. The HBV genotype was determined by direct sequencing and phylogenetic analysis. Of 1292 samples, the distribution of genotype was 514 (39.78%) genotype B, 776 (60.06%) genotype C, 2 (0.16%) genotype D. The results of HBsAg and HBeAg quantification between the Architect and Elecsys assays were significantly correlated (HBsAg: r = 0.939; HBeAg: r = 0.987), independent of HBV genotype and treatment phase. The mean differences between the two methods (the log10 [Elecsys] - the log10 [Architect]) were 0.075 log10 IU/mL and −0.149 log10PE IU/mL in quantifying HBsAg and HBeAg, respectively. This study demonstrates a high correlation between the Elecsys and the Architect assays in quantifying HBsAg and HBeAg, regardless of HBV genotype. Both the two assays can be used to monitor the HBsAg and HBeAg levels in patients with chronic hepatitis B.