Guangzhong Song and Hongyu Jia contributed equally to this work.
Studying the association of microRNA-210 level with chronic hepatitis B progression
Version of Record online: 5 AUG 2013
© 2013 John Wiley & Sons Ltd
Journal of Viral Hepatitis
Volume 21, Issue 4, pages 272–280, April 2014
How to Cite
Song, G., Jia, H., Xu, H., Liu, W., Zhu, H., Li, S., Shi, J., Li, Z., He, J. and Chen, Z. (2014), Studying the association of microRNA-210 level with chronic hepatitis B progression. Journal of Viral Hepatitis, 21: 272–280. doi: 10.1111/jvh.12138
- Issue online: 6 MAR 2014
- Version of Record online: 5 AUG 2013
- Manuscript Accepted: 23 MAY 2013
- Manuscript Received: 26 FEB 2013
- 12-5 State S&T Projects of China. Grant Numbers: 2012ZX10002007, 2013ZX10002001
- chronic hepatitis B;
- hepatitis progression;
We studied the relationship between hypoxia and microRNA-210 (miR-210) levels, the miR-210 levels in patients with hepatitis B and the roles of miR-210 in liver inflammation. We used the concanavalin A (Con A) murine hepatitis model and inflammation, hypoxia and miR-210 levels were examined. In these patients, we studied serum miR-210 levels and clinical indexes related to hepatitis in 90 patients with different stages of chronic hepatitis B and 30 controls. Two functional assays of miR-210 in vitro under hypoxic condition were conducted. The animal experiments indicated that the liver and serum miR-210 levels significantly increased with liver hypoxia and inflammation. In humans, serum miR-210 levels enhanced with hepatitis severity and were related to serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and prothrombin activity (PTA) levels. The miR-210 functional assays showed that miR-210 elevation might be related to the decreases in HepG2.2.15 cell dehydrogenase activity and HBV replication under hypoxic conditions. Because the liver inflammation causes liver hypoxia which also results in liver and serum miR-210 level elevation, the serum miR-210 level may serve as a molecular biomarker for the severity of hepatitis and increases in liver miR-210 that we see may be a response of hepatocytes to hypoxia during hepatitis progression.