This work was performed at the Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation
Effects of Dexamethasone-21-Isonicotinate on Peripheral Insulin Action in Dairy Cows 5 days after Surgical Correction of Abomasal Displacement
Article first published online: 29 NOV 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 1, pages 200–206, January/February 2013
How to Cite
Kusenda, M., Kaske, M., Piechotta, M., Locher, L., Starke, A., Huber, K. and Rehage, J. (2013), Effects of Dexamethasone-21-Isonicotinate on Peripheral Insulin Action in Dairy Cows 5 days after Surgical Correction of Abomasal Displacement. Journal of Veterinary Internal Medicine, 27: 200–206. doi: 10.1111/jvim.12010
- Issue published online: 11 JAN 2013
- Article first published online: 29 NOV 2012
- Manuscript Accepted: 26 SEP 2012
- Manuscript Revised: 30 JUN 2012
- Manuscript Received: 31 DEC 2011
- Hyperinsulinemic-euglycemic clamp;
- Insulin sensitivity;
Dexamethasone frequently is used for treatment of ketosis in dairy cows, but its effects are not fully understood.
Dexamethasone treatment affects whole body insulin sensitivity.
Twelve German Holstein cows, 2–4 weeks postpartum, 5 days after omentopexy to correct left abomasal displacement.
Randomized, blinded, case-control study. Treatment with dexamethasone-21-isonicotinate (DG; 40 μg/kg IM; n = 6) or saline (control group [CG], 15 mL IM, n = 6) on day 0 (d0). Blood samples were obtained before (d0) and after treatment (d1 and d2), and analyzed for glucose, insulin, and nonesterified fatty acid (NEFA) concentrations. Hepatic triglycerides (TAG) were measured in liver samples taken on d0 and d2. Five consecutive hyperinsulinemic-euglycemic clamps (HEC-I-V; insulin dosages: 0.1, 0.5, 2, 5, 10 mU/kg/min, respectively) were performed on d1 and steady state glucose infusion rate (SSGIR), insulin concentration (SSIC), insulin sensitivity index (ISI = SSGIR/SSIC), and plasma NEFA concentration (SSNEFA) were assessed.
Compared with CG-cows, DG-cows on d1 had higher plasma glucose (P = .004) and insulin (P < .001) concentrations, decreased SSGIR (HEC-II, P = .002; HEC-IV, P = .033), ISI (HEC-I, P < .015; HEC-II, P = .004), and insulin-stimulated decrease in SSNEFA (HEC-II, P = .006; HEC-III, P = .01; HEC-IV, P = .003; HEC-V, P = .011). Decrease in hepatic TAG content in DG-cows was higher compared with CG-cows (P < .001).
Conclusions and Clinical Importance
Dexamethasone decreases whole body insulin sensitivity and affects glucose and lipid metabolism in early lactating dairy cows.