• Open Access

A Single-Blood-Sample Method Using Inulin for Estimating Feline Glomerular Filtration Rate

Authors


Corresponding author: Kazuhisa Furuhama, Department of Veterinary Basic Medicine, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan; e-mail: furuhama@iwate-u.ac.jp.

Abstract

Background

Application of a multisample method using inulin to estimate glomerular filtration rate (GFR) in cats is cumbersome.

Objectives

To establish a simplified procedure to estimate GFR in cats, a single-blood-sample method using inulin was compared with a conventional 3-sample method.

Animals

Nine cats including 6 clinically healthy cats and 3 cats with spontaneous chronic kidney disease.

Methods

Retrospective study. Inulin was administered as an intravenous bolus at 50 mg/kg to cats, and blood was collected at 60, 90, and 120 minutes later for the 3-sample method. Serum inulin concentrations were colorimetrically determined by an autoanalyzer method. The GFR in the single-blood-sample method was calculated from the dose injected, serum concentration, sampling time, and estimated volume of distribution on the basis of the data of the 3-sample method.

Results

An excellent correlation was observed (r = 0.99, P = .0001) between GFR values estimated by the single-blood-sample and 3-sample methods.

Conclusions and Clinical Importance

The single-blood-sample method using inulin provides a practicable and ethical alternative for estimating glomerular filtration rate in cats.

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