The study was supported in part by the Hunting Retriever Club Foundation, Boston Scientific (Natwick, MA) and Olympus America (Center Valley, PA).
Effect of Endoscopic Forceps on Quality of Duodenal Mucosal Biopsy in Healthy Dogs
Article first published online: 18 APR 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 3, pages 456–461, May/June 2013
How to Cite
Goutal-Landry, C.M., Mansell, J., Ryan, K.A. and Gaschen, F.P. (2013), Effect of Endoscopic Forceps on Quality of Duodenal Mucosal Biopsy in Healthy Dogs. Journal of Veterinary Internal Medicine, 27: 456–461. doi: 10.1111/jvim.12085
A research abstract was presented at the 2012 ACVIM Forum, New Orleans, Louisiana, May 30 – June 2
- Issue published online: 9 MAY 2013
- Article first published online: 18 APR 2013
- Manuscript Accepted: 7 MAR 2013
- Manuscript Revised: 12 DEC 2012
- Manuscript Received: 24 JUL 2012
- Hunting Retriever Club Foundation
Limited data exist regarding influence of endoscopic forceps on duodenal mucosal biopsy quality and adequacy for histologic examination/assessment in dogs.
Hypothesizing that larger forceps would procure superior specimens, we evaluated effect of 6 disposable forceps on duodenal biopsy weight, depth, crush artifact, and adequacy for histologic examination/assessment.
Seventeen healthy adult dogs.
Prospective study. Two operators each obtained 4 duodenal specimens from each dog with each forceps. Lightest sample discarded. One pathologist evaluated blindly other 3 specimens. A total of 612 specimens evaluated. Results analyzed by one-way ANOVA of forceps effects with dog as blocking factor. Posthoc pairwise comparisons examined with Tukey's test when indicated.
Biopsies performed with large capacity forceps heavier (10.56 ± 0.90 and 11.6 ± 0.62 mg (mean ± SD) versus 5.55 ± 0.53 to 8.61 ± 0.49; P < .0001) and adequacy for histologic examination/assessment superior to standard oval and ‘pediatric’ (scores 2.52 ± 0.41 and 2.58 ± 0.37 versus 2.08 ± 0.33 and 2.14 ± 0.29; P < .0001). No statistically significant difference in depth scores. Large capacity forceps with spike associated with less crush artifact than all smaller forceps (scores 1.19 ± 0.16 versus 1.38 ± 0.21 to 1.52 ± 0.21; P < .0001). In same size forceps, presence of spike had no effect on crush artifact and adequacy for histologic examination/assessment (P < .0001).
Conclusions and Clinical Importance
Large capacity forceps are superior, providing higher quality and greater numbers of samples achieving adequacy for histologic examination/assessment. Choice of endoscopic biopsy forceps for duodenal samples influences sample volume and diagnostic utility.