Data from this study was presented as an abstract at the 2012 American College of Veterinary Internal Medicine Forum, New Orleans, LA.
Phylogenetic Diversity of Bacteria Isolated from Sick Dogs Using the BAPGM Enrichment Culture Platform
Article first published online: 6 MAY 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 4, pages 854–861, July/August 2013
How to Cite
Davenport, A.C., Mascarelli, P.E., Maggi, R.G. and Breitschwerdt, E.B. (2013), Phylogenetic Diversity of Bacteria Isolated from Sick Dogs Using the BAPGM Enrichment Culture Platform. Journal of Veterinary Internal Medicine, 27: 854–861. doi: 10.1111/jvim.12094
- Issue published online: 15 JUL 2013
- Article first published online: 6 MAY 2013
- Manuscript Accepted: 13 MAR 2013
- Manuscript Revised: 19 FEB 2013
- Manuscript Received: 9 NOV 2012
- American College of Veterinary Internal Medicine Foundation
- Bayer Animal Health
- State of North Carolina
Bartonella alpha-Proteobacteria growth medium (BAPGM) enrichment culture has proven useful for documenting Bartonella species infection and has facilitated growth of other fastidious bacteria from human samples.
To report non-Bartonella bacterial isolates obtained from canine samples cultured using BAPGM enrichment culture.
Between 2004 and 2008, 695 specimens from 513 dogs were tested by the NCSU-IPRL using the BAPGM enrichment culture. Over the same period of time, blood samples from 270 dogs were cultured by the NCSU-CML using Bactec-Plus Aerobic/F media.
BAPGM isolates were characterized using Bartonella genus primers and 16S rDNA primers followed by DNA sequencing. NCSU medical records were retrospectively reviewed. Blood culture results from the NCSU-CML were compared with BAPGM blood culture results.
Seventy-nine non-Bartonella isolates were obtained from 69/513 dogs. The most commonly isolated phylum was Proteobacteria (48.1%) with alpha-Proteobacteria being the most commonly isolated class. Staphylococcus and Sphingomonas were the most commonly isolated genera. The majority of the remaining isolates were bacteria that are rarely isolated from canine samples. Comparison of NCSU-CML and IPRL (BAPGM) blood culture isolates showed alpha-Proteobacteria were isolated more often from BAPGM.
Conclusions and Clinical Importance
Use of insect cell culture enrichment medium, such as BAPGM, appears to enhance the growth of alpha-Proteobacteria, but also results in isolation of non-alpha-Proteobacteria from sick dogs. Future studies are needed to elucidate the utility of BAPGM and other “nonconventional” growth media and methods for isolation of fastidious organisms and to determine if these organisms play a causal role in disease development.