Evaluation of Hemostatic Activity of Canine Frozen Plasma for Transfusion by Thromboelastography
Version of Record online: 10 MAY 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 4, pages 964–969, July/August 2013
How to Cite
Urban, R., Guillermo Couto, C. and Cristina Iazbik, M. (2013), Evaluation of Hemostatic Activity of Canine Frozen Plasma for Transfusion by Thromboelastography. Journal of Veterinary Internal Medicine, 27: 964–969. doi: 10.1111/jvim.12097
- Issue online: 15 JUL 2013
- Version of Record online: 10 MAY 2013
- Manuscript Accepted: 14 MAR 2013
- Manuscript Revised: 27 NOV 2012
- Manuscript Received: 11 AUG 2012
- Greyhound Health and Wellness Program Development Funds
In humans, fresh frozen plasma (FFP) loses factor V and VIII activities after 1 year. It then becomes frozen plasma (FP), and theoretically is unsuitable for use in patients with coagulopathies. These findings have not been reported for dogs.
Canine FP is hemostatically active after 5 years of storage.
Fresh plasma (Group FsP; n = 15) and 5-year-old FP (Group FzP; n = 10) from blood bank donors.
Group FsP and Group FzP samples were evaluated by thromboelastography (TEG), one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), fibrinogen, and antithrombin. Fresh plasma (n = 6) and a subset of Group FzP (n = 8) were evaluated for clotting factor activities (V, VIII, IX, X). A 2nd experiment using short-term storage of thawed FP under suboptimal conditions (refrigerated [4°C] or refrozen [−20°C]) by TEG was performed to simulate general practice storage capabilities.
Group FzP had shorter reaction time (P = .0007) and larger angle (P = .0004) compared with Group FsP by TEG, suggesting hypercoaguability. Factor VIII and X activities were lower in Group FzP (P = .02 and .005, respectively). Fibrinogen, OSPT, and APTT were significantly lower or longer for Group FzP than Group FsP (P < .05), but most values remained within reference intervals for dogs.
Conclusions and Clinical Importance
Five-year-old canine FP stored at −30°C is hemostatically active and should be clinically evaluated in patients with coagulopathies. If active, the monetary savings of using older plasma will be substantial.