Animal work was performed in the Comparative Internal Medicine Laboratory at the University of Missouri. The ET assay was performed in the Clinical Sciences Laboratory at the Tufts Cummings School of Veterinary Medicine. This study was presented in abstract form at the 2010 ACVIM Forum, Anaheim, CA
Endothelin-1 Concentrations in Bronchoalveolar Lavage Fluid of Cats with Experimentally Induced Asthma
Article first published online: 10 JUN 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 4, pages 982–984, July/August 2013
How to Cite
Sharp, C.R., Lee-Fowler, T.M. and Reinero, C.R. (2013), Endothelin-1 Concentrations in Bronchoalveolar Lavage Fluid of Cats with Experimentally Induced Asthma. Journal of Veterinary Internal Medicine, 27: 982–984. doi: 10.1111/jvim.12119
- Issue published online: 15 JUL 2013
- Article first published online: 10 JUN 2013
- Manuscript Accepted: 24 APR 2013
- Manuscript Revised: 28 FEB 2013
- Manuscript Received: 5 SEP 2010
- Airway inflammation;
- Animal model;
There is a need for biomarkers for diagnosis, therapeutic monitoring, and prognosis for asthma in cats. Endothelin-1 (ET-1) is implicated in the pathogenesis of inflammatory airway diseases in other species but not the cat.
To conduct a prospective experimental study to show that experimentally asthmatic cats, but not control cats without airway inflammation, would have increased concentrations of ET in BALF.
Eleven healthy, adult research cats.
Prospective experimental study. Six healthy cats without airway inflammation were used as controls. Asthma was induced using Bermuda grass allergen (BGA) in 5 cats. Collection of BALF for total nucleated cell and differential counts was performed. The concentration of ET-1 in cell-free BALF samples was determined. Data were analyzed using a Mann–Whitney U-test with P < .05 considered significant.
The median [range] BALF total cell numbers, eosinophil numbers, and eosinophil percentages were significantly higher in the cats following experimental induction of asthma (1,870 cells/μL [1,450–3,440], 711 cells/μL [356–1,686] and 38% [20–49]) compared to baseline control parameters (462 cells/μL [239–780], 18 cells/μL [18–62] and 3.5% [0–8]) (P < .01). The median [range] BALF ET concentration was also significantly higher after induction of asthma (1.393 fmol/mL[0.977–2.247]) compared to healthy control cats (0.83250 fmol/mL [0.625–1.038]) (P = .012).
Conclusions and Clinical Importance
This study suggests that BAL ET-1 concentration can be used to differentiate normal cats from those with experimentally induced asthma. If the same holds true for cats with naturally developing asthma, BAL ET-1 may prove a useful diagnostic biomarker for asthma.