Presented in part as an abstract at the 1st European joint congress of EFCC and UEMS: Laboratory Medicine in Health Care, Lisabon, September 2010, and 14th Congress of International Society for Animal Clinical Pathology; Portland, November 2010.
Markers of Coagulation Activation, Endothelial Stimulation, and Inflammation in Dogs with Babesiosis
Version of Record online: 22 JUL 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 5, pages 1172–1178, September/October 2013
How to Cite
Barić Rafaj, R., Kuleš, J., Selanec, J., Vrkić, N., Zovko, V., Zupančič, M., Trampuš Bakija, A., Matijatko, V., Crnogaj, M. and Mrljak, V. (2013), Markers of Coagulation Activation, Endothelial Stimulation, and Inflammation in Dogs with Babesiosis. Journal of Veterinary Internal Medicine, 27: 1172–1178. doi: 10.1111/jvim.12146
- Issue online: 13 SEP 2013
- Version of Record online: 22 JUL 2013
- Manuscript Accepted: 6 JUN 2013
- Manuscript Revised: 30 APR 2013
- Manuscript Received: 18 OCT 2012
- Project of Ministry of Science and Technologies. Grant Number: 053-0532266-2220
- Inflammatory markers;
- Intercellular adhesion molecule;
- von Willebrand factor
Babesia infections in dogs can result in a wide range of clinical and laboratory presentations, including coagulopathy. Expression of intercellular adhesion molecule-1 (ICAM-1) and von Willebrand factor (vWF) in dogs with babesiosis is unknown.
Whether inflammation in babesiosis triggers activation of ICAM-1 and the coagulation system.
Twelve and 10 dogs with naturally occurring babesiosis before and after antiparasitic treatment, respectively, were compared with 10 healthy dogs.
In this prospective study, diagnosis was made by blood smear examination and confirmed by PCR. C-reactive protein (CRP), soluble intercellular adhesion molecule 1 (sICAM-1), and von Willebrand factor (vWF) levels were measured by a canine ELISA kit, fibrinogen (FIB) and factor VIII activity levels were measured by coagulometric methods, and blood cell counts (WBC, RBC, PLT) were determined with an automatic analyzer.
Compared to healthy dogs, the CRP, sICAM-1, and FIB concentrations were significantly increased before therapy and remained high for 3 days after therapy in dogs with babesiosis. vWF activity was significantly decreased in dogs with babesiosis before treatment. FVIII activity did not differ between dogs with babesiosis and healthy dogs. WBC; RBC and PLT were significantly lower before treatment and normalized by 3 days after treatment.
Conclusion and Clinical Importance
A proinflammatory condition in babesiosis appears to influence endothelial dysfunction and hemostatic activity. Although clearly beneficial for the parasite, sequestered blood cells can obstruct blood flow in small vessels, promote an inflammatory state, and could increase the severity of babesiosis.