• Open Access

Pharmacokinetics and Relative Bioavailability of d-Penicillamine in Fasted and Nonfasted Dogs

Authors


  • The study was conducted at the Michigan State University College of Veterinary Medicine. Sample analysis was performed at the Michigan State University Diagnostic Center for Population and Animal Health.

Corresponding author: D.K. Langlois, Veterinary Teaching Hospital, Michigan State University, 736 Wilson Road, East Lansing, MI 48824-1314; email: langlo21@cvm.msu.edu.

Abstract

Background

d-Penicillamine is the most commonly used copper-chelating agent in the treatment of copper-associated hepatitis in dogs. Response to therapy can be variable, and there is a lack of pharmacokinetic information available for dogs. Coadministering the drug with food to alleviate vomiting has been recommended for dogs, which contradicts recommendations for drug administration to humans.

Hypothesis

Coadministration of d-penicillamine with food decreases relative bioavailability and maximum plasma drug concentrations (Cmax) in dogs.

Animals

Nine purpose-bred dogs with a median body weight of 17.0 kg.

Methods

Dogs received d-penicillamine (12.5 mg/kg PO) fasted and with food in a randomized, crossover design. Blood samples were collected before and 0.25, 0.5, 1, 2, 3, 4, 8, 12, and 24 hours after dosing. Total d-penicillamine concentrations were measured using liquid chromatography coupled with tandem quadrupole mass spectrometry. Pharmacokinetic parameters were calculated for each dog.

Results

Two fasted dogs (22%) vomited after receiving d-penicillamine. Mean Cmax ± standard deviation (SD) was 8.7 ± 3.1 μg/mL (fasted) and 1.9 ± 1.6 μg/mL (fed). Mean area under the plasma concentration curve ± SD was 16.9 ± 5.9 μg/mL·h (fasted) and 4.9 ± 3.4 μg/mL·h (fed). There were significant reductions in relative bioavailability and Cmax in fed dogs (P < .001).

Conclusions and Clinical Importance

Coadministration of d-penicillamine with food significantly decreases plasma drug concentrations in dogs. Decreased drug exposure could result in decreased copper chelation efficacy, prolonged therapy, additional cost, and greater disease morbidity. Administration of d-penicillamine with food cannot be categorically recommended without additional studies.

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