• Open Access

Lymphoma Immunophenotype of Dogs Determined by Immunohistochemistry, Flow Cytometry, and Polymerase Chain Reaction for Antigen Receptor Rearrangements

Authors

  • L. Thalheim,

    1. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
    Search for more papers by this author
  • L.E. Williams,

    1. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
    2. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC
    Search for more papers by this author
  • L.B. Borst,

    1. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC
    2. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
    Search for more papers by this author
  • J.E. Fogle,

    1. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC
    2. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
    Search for more papers by this author
  • S.E. Suter

    Corresponding author
    1. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
    2. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC
    3. Lineberger Comprehensive Cancer Center, Chapel Hill, NC
    • Corresponding author: S.E. Suter VMD, PhD, ACVIM (Oncology), Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1501 William Moore Drive, CVM Research #308, Raleigh, NC 27607; e-mail: steven_suter@ncsu.edu

    Search for more papers by this author

  • The results of this study were presented at the 2011 Veterinary Cancer Society meeting and the 2012 ACVIM meeting. This work was performed at the North Carolina State College of Veterinary Medicine Veterinary Health Complex

Abstract

Background

Immunohistochemistry (IHC), flow cytometry (FC), and PCR for antigen receptor rearrangements (PARR) are 3 widely utilized tests to determine immunophenotype in dogs with lymphoma (LSA).

Objectives

This study evaluated the ability of FC and PARR to correctly predict immunophenotype as defined by IHC and to determine the level of agreement among the 3 tests.

Animals

Sixty-two dogs with lymphoma.

Methods

Retrospective study. Medical records were searched to identify dogs with LSA that had concurrent IHC, FC, and PARR performed. Immunophenotype results were categorized as B-cell, T-cell, dual immunophenotype (B- and T-cell), or indeterminate. The results of FC and PARR were evaluated for correctly classifying B- and T-cell LSA as compared with IHC. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were evaluated in addition to concordance between each test.

Results

The sensitivity of FC was significantly higher than PARR for both B-cell (91% versus 67%; P < 0.0072) and T-cell (100% versus 75%; P < 0.0312) LSA. The percent agreement between FC and IHC was 94%, between PARR and IHC was 69%, between FC and PARR was 63%, and among all 3 tests was 63%.

Conclusions and Clinical Importance

Flow cytometry is superior to PARR in correctly predicting immunophenotype when evaluating lymph nodes from dogs already diagnosed with B- or T-cell LSA. If fresh samples are not available for FC, PARR is an acceptable assay for determination of immunophenotype given its high specificity.

Ancillary