The results of this study were presented at the 2011 Veterinary Cancer Society meeting and the 2012 ACVIM meeting. This work was performed at the North Carolina State College of Veterinary Medicine Veterinary Health Complex
Lymphoma Immunophenotype of Dogs Determined by Immunohistochemistry, Flow Cytometry, and Polymerase Chain Reaction for Antigen Receptor Rearrangements
Article first published online: 20 SEP 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 6, pages 1509–1516, November/December 2013
How to Cite
Thalheim, L., Williams, L.E., Borst, L.B., Fogle, J.E. and Suter, S.E. (2013), Lymphoma Immunophenotype of Dogs Determined by Immunohistochemistry, Flow Cytometry, and Polymerase Chain Reaction for Antigen Receptor Rearrangements. Journal of Veterinary Internal Medicine, 27: 1509–1516. doi: 10.1111/jvim.12185
- Issue published online: 13 NOV 2013
- Article first published online: 20 SEP 2013
- Manuscript Accepted: 6 AUG 2013
- Manuscript Revised: 12 JUL 2013
- Manuscript Received: 3 APR 2013
- UNC Lineberger Comprehensive Cancer Center UCRF
- NCSU Center for Comparative Medicine and Translational Research
- Flow cytometry;
- Lymph nodes;
Immunohistochemistry (IHC), flow cytometry (FC), and PCR for antigen receptor rearrangements (PARR) are 3 widely utilized tests to determine immunophenotype in dogs with lymphoma (LSA).
This study evaluated the ability of FC and PARR to correctly predict immunophenotype as defined by IHC and to determine the level of agreement among the 3 tests.
Sixty-two dogs with lymphoma.
Retrospective study. Medical records were searched to identify dogs with LSA that had concurrent IHC, FC, and PARR performed. Immunophenotype results were categorized as B-cell, T-cell, dual immunophenotype (B- and T-cell), or indeterminate. The results of FC and PARR were evaluated for correctly classifying B- and T-cell LSA as compared with IHC. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were evaluated in addition to concordance between each test.
The sensitivity of FC was significantly higher than PARR for both B-cell (91% versus 67%; P < 0.0072) and T-cell (100% versus 75%; P < 0.0312) LSA. The percent agreement between FC and IHC was 94%, between PARR and IHC was 69%, between FC and PARR was 63%, and among all 3 tests was 63%.
Conclusions and Clinical Importance
Flow cytometry is superior to PARR in correctly predicting immunophenotype when evaluating lymph nodes from dogs already diagnosed with B- or T-cell LSA. If fresh samples are not available for FC, PARR is an acceptable assay for determination of immunophenotype given its high specificity.