The work was done at in Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey
Evaluation of 4 Point-of-Care Units for the Determination of Blood l-Lactate Concentration in Cattle
Article first published online: 1 OCT 2013
Copyright © 2013 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 27, Issue 6, pages 1596–1603, November/December 2013
How to Cite
Karapinar, T., Kaynar, O., Hayirli, A. and Kom, M. (2013), Evaluation of 4 Point-of-Care Units for the Determination of Blood l-Lactate Concentration in Cattle. Journal of Veterinary Internal Medicine, 27: 1596–1603. doi: 10.1111/jvim.12204
- Issue published online: 13 NOV 2013
- Article first published online: 1 OCT 2013
- Manuscript Accepted: 20 AUG 2013
- Manuscript Revised: 19 JUN 2013
- Manuscript Received: 12 SEP 2012
- The Scientific and Technological Research Council of Turkey. Grant Number: 111O045
- Portable lactate meter;
Despite being used commonly in bovine medicine, information on reliability of point-of-care (POC) lactate meters is limited.
To determine the validity of 4 commercially available POC lactate meters in cattle.
Cattle with various diseases (n = 120).
Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma l-lactate concentration was measured by the enzymatic-colorimetric method (ELISA). Data were subjected to Friedman's test for comparison, Passing-Bablok regression and Bland-Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity (Se) and specificity (Sp).
The POC lactate meters were highly correlated with ELISA (r = 0.98–0.99) despite disagreements among units. When regressed on ELISA, blood l-lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i-STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7–98.6% at a plasma l-lactate cut-off concentration of 4 mmol/L. i-STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit (y = −0.13 + 1.04X) yielding the lowest bias (−6.6%) as well as the highest Se (100%) and Sp (98.6%).
Conclusions and Clinical Importance
Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i-STAT/Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i-STAT provided the most accurate and precise results.