• Open Access

Evaluation of 4 Point-of-Care Units for the Determination of Blood l-Lactate Concentration in Cattle

Authors


  • The work was done at in Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey

Abstract

Background

Despite being used commonly in bovine medicine, information on reliability of point-of-care (POC) lactate meters is limited.

Objective

To determine the validity of 4 commercially available POC lactate meters in cattle.

Animals

Cattle with various diseases (n = 120).

Methods

Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma l-lactate concentration was measured by the enzymatic-colorimetric method (ELISA). Data were subjected to Friedman's test for comparison, Passing-Bablok regression and Bland-Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity (Se) and specificity (Sp).

Results

The POC lactate meters were highly correlated with ELISA (r = 0.98–0.99) despite disagreements among units. When regressed on ELISA, blood l-lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i-STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7–98.6% at a plasma l-lactate cut-off concentration of 4 mmol/L. i-STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit (y = −0.13 + 1.04X) yielding the lowest bias (−6.6%) as well as the highest Se (100%) and Sp (98.6%).

Conclusions and Clinical Importance

Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i-STAT/Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i-STAT provided the most accurate and precise results.

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